HaemoScan CH50 Test (Classical Pathway Complement Activity)

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產(chǎn)品名稱: HaemoScan CH50 Test (Classical Pathway Complement Activity)

產(chǎn)品型號: K002

產(chǎn)品展商: HaemoScan

產(chǎn)品文檔: 無相關(guān)文檔

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HaemoScan CH50 Test (Classical Pathway Complement Activity) 的詳細(xì)介紹

CH50 測試（經(jīng)典途徑補(bǔ)體活性）

經(jīng)典途徑方法（CH50）基于在 Ca++ 和 Mg++ 存在下裂解致敏綿羊紅細(xì)胞。
當(dāng)懷疑補(bǔ)體缺失或缺乏時(shí)，它可以作為篩查試驗(yàn)。該測試對途徑成分的減少、缺失和惰性敏感。該方法也適用于評估醫(yī)藥產(chǎn)品對補(bǔ)體成分抑制或消耗的影響。

◆ 應(yīng)用
經(jīng)典途徑補(bǔ)體活性的測定
◆ 原理
將紅細(xì)胞懸液與連續(xù)稀釋的血清或血漿在 37 °C 下孵育 30 分鐘。
補(bǔ)體系統(tǒng)的活性導(dǎo)致溶血。孵育后，將樣品離心以獲得上清液。上清液中游離血紅蛋白的濃度與補(bǔ)體活性成正比，使用分光光度計(jì)在 415 nm 波長下測量。繪制血漿稀釋因子與溶血程度的關(guān)系可以計(jì)算 CH50。（即稀釋血清以獲得 50% 紅細(xì)胞的裂解）

該試劑盒旨在通過經(jīng)典途徑測量少量樣品（25 μL）中的補(bǔ)體活性，可在 96 孔（圓底）微孔板上執(zhí)行。該測定也可使用微量離心管進(jìn)行。

◆ 保存和穩(wěn)定性
產(chǎn)品在 4°C 下可穩(wěn)定保存至少 6 周

◆ 參考文獻(xiàn)

1. Pinnapireddy SR , Giselbrecht J , Strehlow B , Janich C , Husteden C , Meister A , Loppnow H , Sedding D , Erdmann F , Hause G , Brezesinski G , Groth T , Langner A , Bakowsky U , W?lk C . A triple chain polycationic peptide-mimicking amphiphile - efficient DNA-transfer without co-lipids. Biomater Sci. 2019 Dec 17; 8(1):232-249.
2. Giselbrecht J, Wiedemann S, Reddy Pinnapireddy S, Goergen N, Loppnow H, Sedding D, Erdmann F, Bakowsky U, Hause G, Lúcio M, Langner A, W?lk C. Nucleic acid carrier composed of a branched fatty acid lysine conjugate-Interaction studies with blood components. Colloids Surf B Biointerfaces. 2019 Dec 1;184:110547.
3. Garaulet G, Lazcano JJ, Alarcón H, de Frutos S, Martínez-Torrecuadrada JL, Rodríguez A. Display of the Albumin-Binding Domain in the Envelope Improves Lentiviral Vector Bioavailability. Hum Gene Ther Methods. 2017 Dec; 28(6):340-351.
4. Akhouri RR, Goel S, Furusho H, Skoglund U, Wahlgren M. Architecture of Human IgM in Complex with P. falciparum Erythrocyte Membrane Protein 1. Cell Rep. 2016 Feb 2; 14(4):723-736.
5. Misra SK, Chang HH, Mukherjee P, Tiwari S, Ohoka A, Pan D. Regulating Biocompatibility of Carbon Spheres via Defined Nanoscale Chemistry and a Careful Selection of Surface Functionalities. Sci Rep. 2015 Oct 14;5:14986.
6. Mukherjee P, Misra SK, Gryka MC, Chang HH, Tiwari S, Wilson WL, Scott JW, Bhargava R, Pan D. Tunable Luminescent Carbon Nanospheres with Well-Defined Nanoscale Chemistry for Synchronized Imaging and Therapy. Small. 2015 Sep; 11(36):4691-703.
7. Luo J, Lindstrom J. Antigen-specific immunotherapeutic vaccine for experimental autoimmune myasthenia gravis. J Immunol. 2014 Nov 15; 193(10):5044-55.

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