Product Gene Name
anti-WRN antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q14191
Immunogen
Recombinant Protein
Other Notes
Small volumes of anti-WRN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-WRN antibody
Polyclonal
Applications Tested/Suitable for anti-WRN antibody
Western Blot (WB)
Western Blot (WB) of anti-WRN antibody
Western blot analysis of extracts of various cell lines, using WRN antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
NCBI/Uniprot data below describe general gene information for WRN. It may not necessarily be applicable to this product.
NCBI Accession #
Q14191.2
[Other Products]
UniProt Primary Accession #
Q14191
[Other Products]
UniProt Secondary Accession #
A1KYY9[Other Products]
UniProt Related Accession #
Q14191[Other Products]
NCBI Official Full Name
Werner syndrome ATP-dependent helicase
NCBI Official Synonym Full Names
Werner syndrome, RecQ helicase-like
NCBI Official Symbol
WRN??[Similar Products]
NCBI Official Synonym Symbols
RECQ3; RECQL2; RECQL3
??[Similar Products]
NCBI Protein Information
Werner syndrome ATP-dependent helicase; exonuclease WRN; recQ protein-like 2; DNA helicase, RecQ-like type 3
UniProt Protein Name
Werner syndrome ATP-dependent helicase
UniProt Synonym Protein Names
DNA helicase, RecQ-like type 3; RecQ3; Exonuclease WRN (EC:3.1.-.-); RecQ protein-like 2
Protein Family
Werner syndrome ATP-dependent helicase
UniProt Gene Name
WRN??[Similar Products]
UniProt Synonym Gene Names
RECQ3; RECQL2; RecQ3??[Similar Products]
UniProt Entry Name
WRN_HUMAN
NCBI Summary for WRN
This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]
UniProt Comments for WRN
WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating s for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.
Protein type: Nucleolus; DNA-binding; DNA repair, damage; Cell cycle regulation; Helicase; EC 3.6.4.12
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: nucleoplasm; MutLalpha complex; centrosome; nucleolus
Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; ATPase activity; magnesium ion binding; 3'-5' DNA helicase activity; bubble DNA binding; helicase activity; four-way junction helicase activity; Y-form DNA binding; ATP-dependent DNA helicase activity; protein binding; DNA helicase activity; ATP-dependent 3'-5' DNA helicase activity; DNA binding; manganese ion binding; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; ATP binding
Biological Process: nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; multicellular organismal aging; cell aging; cellular response to starvation; replicative cell aging; response to UV-C; DNA duplex unwinding; DNA recombination; replication fork processing; regulation of apoptosis; DNA synthesis during DNA repair; base-excision repair; double-strand break repair; response to oxidative stress; regulation of growth rate; DNA replication; response to DNA damage stimulus; telomere maintenance; DNA metabolic process; aging
Disease: Werner Syndrome
Research Articles on WRN
1. WRN and BLM act epistatically with DNA2 to promote the long-range resection of double strand break ends in human cells.
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