Full Product Name
Goat anti-GPSM2 Antibody
Product Synonym Names
GPSM2; G-protein signalling modulator 2 (AGS3-like, C. elegans); HGNC: 29501; LGN; Pins; LGN protein; GPSM2 antibody; G-protein signalling modulator 2 (AGS3-like; C. elegans) antibody; HGNC: 29501 antibody; LGN antibody; Pins antibody; LGN protein antibody
Product Gene Name
anti-GPSM2 antibody
[Similar Products]
Antibody/Peptide Pairs
GPSM2 peptide (MBS426838) is used for blocking the activity of GPSM2 antibody (MBS421246)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
NRLKGKKYKT NSSTK
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-NRLKGKKYKTNSSTK, from the internal region of the protein sequence according to NP_037428.2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-GPSM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GPSM2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-GPSM2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot (WB) of anti-GPSM2 antibody
(0. 01ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for GPSM2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037428.2
[Other Products]
NCBI GenBank Nucleotide #
NM_013296.3
[Other Products]
UniProt Secondary Accession #
Q5T1N8; Q6IBL7; Q8N0Z5[Other Products]
UniProt Related Accession #
P81274[Other Products]
Molecular Weight
76,662 Da
NCBI Official Full Name
G-protein signaling modulator 2 (AGS3-like, C. elegans)
NCBI Official Synonym Full Names
G-protein signaling modulator 2
NCBI Official Symbol
GPSM2??[Similar Products]
NCBI Official Synonym Symbols
LGN; CMCS; PINS; DFNB82
??[Similar Products]
NCBI Protein Information
G-protein-signaling modulator 2
UniProt Protein Name
G-protein-signaling modulator 2
UniProt Synonym Protein Names
Mosaic protein LGN
Protein Family
G-protein-signaling modulator
UniProt Gene Name
GPSM2??[Similar Products]
UniProt Synonym Gene Names
LGN??[Similar Products]
UniProt Entry Name
GPSM2_HUMAN
NCBI Summary for GPSM2
The protein encoded by this gene belongs to a family of proteins that modulate activation of G proteins, which transduce extracellular signals received by cell surface receptors into integrated cellular responses. The N-terminal half of this protein contains 10 copies of leu-gly-asn (LGN) repeat, and the C-terminal half contains 4 GoLoco motifs, which are involved in guanine nucleotide exchange. This protein may play a role in neuroblast division and in the development of normal hearing. Mutations in this gene are associated with autosomal recessive nonsyndromic deafness (DFNB82). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Comments for GPSM2
GPSM2: Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions. Interacts with LLGL2. Interacts with INSC/inscuteable and probably with F2RL2. Ubiquitously expressed. Belongs to the GPSM family.
Protein type: G protein regulator, misc.
Chromosomal Location of Human Ortholog: 1p13.3
Cellular Component: apical part of cell; cell cortex; cytoplasm
Molecular Function: G-protein alpha-subunit binding; GDP-dissociation inhibitor activity; identical protein binding; nucleotide binding; protein binding
Biological Process: establishment of mitotic spindle orientation; G-protein coupled receptor protein signaling pathway; maintenance of centrosome localization; mitotic spindle organization and biogenesis; regulation of catalytic activity
Disease: Chudley-mccullough Syndrome
Research Articles on GPSM2
1. A crystal structure of Frmpd4-bound LGN in an oxidized form is also reported, although oxidation does not appear to strongly affect the interaction with Frmpd4.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
