Product Name
GPSM2, siRNA
Full Product Name
GPSM2 siRNA (Mouse)
Product Synonym Names
LGN; PINS; G-protein-signaling modulator 2; Pins homolog
Product Gene Name
GPSM2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8VDU0
Specificity
GPSM2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GPSM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GPSM2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GPSM2 sirna
siRNA to inhibit GPSM2 expression using RNA interference
Applications Tested/Suitable for GPSM2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GPSM2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_083798.2
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NCBI GenBank Nucleotide #
NM_029522.2
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UniProt Primary Accession #
Q8VDU0
[Other Products]
UniProt Secondary Accession #
Q8BLX3[Other Products]
UniProt Related Accession #
Q8VDU0[Other Products]
Molecular Weight
75,591 Da
NCBI Official Full Name
G-protein-signaling modulator 2
NCBI Official Synonym Full Names
G-protein signalling modulator 2 (AGS3-like, C. elegans)
NCBI Official Symbol
Gpsm2??[Similar Products]
NCBI Official Synonym Symbols
LGN; Pins; 6230410J09Rik
??[Similar Products]
NCBI Protein Information
G-protein-signaling modulator 2
UniProt Protein Name
G-protein-signaling modulator 2
UniProt Synonym Protein Names
Pins homolog
Protein Family
G-protein-signaling modulator
UniProt Gene Name
Gpsm2??[Similar Products]
UniProt Synonym Gene Names
Lgn; Pins??[Similar Products]
UniProt Entry Name
GPSM2_MOUSE
UniProt Comments for GPSM2
GPSM2: Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions. Interacts with LLGL2. Interacts with INSC/inscuteable and probably with F2RL2. Ubiquitously expressed. Belongs to the GPSM family.
Protein type: G protein regulator, misc.
Cellular Component: cell; apical part of cell; cytoplasm
Molecular Function: identical protein binding; protein binding; GTPase regulator activity
Biological Process: establishment of mitotic spindle orientation
Research Articles on GPSM2
1. support for the hitherto untested model that Par3-mInsc and Galphai3 act cooperatively to polarize LGN and promote perpendicular divisions
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
