Product Name
EIF1AX, Recombinant Protein
Full Product Name
Recombinant Human EIF1AX Protein
Product Synonym Names
EIF1A; EIF4C; Eukaryotic translation initiation factor 1A X-chromosomal; eIF-1A X isoform; Eukaryotic translation initiation factor 4C; eIF-4C
Product Gene Name
EIF1AX recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHHHHH SSGLVPRGSH MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKAD VILKYNADEA RSLKAYGELP EHAKINETDT FGPGDDDEIQ FDDIGDDDED IDDI
3D Structure
ModBase 3D Structure for P47813
Purity/Purification
Greater than 95% as determined by reducing SDS-PAGE.
Form/Format
Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Other Notes
Small volumes of EIF1AX recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EIF1AX recombinant protein
Recombinant Human EIF1AX Protein is produced by our E.coli expression system and the target gene encoding Met1-Ile144 is expressed with a 6His tag at the N-terminus.
Applications Tested/Suitable for EIF1AX recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
NCBI/Uniprot data below describe general gene information for EIF1AX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001403.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001412.3
[Other Products]
UniProt Primary Accession #
P47813
[Other Products]
UniProt Secondary Accession #
Q0VGC2; Q5JPS5; Q5JPS6; B2R5U5[Other Products]
UniProt Related Accession #
P47813[Other Products]
Molecular Weight
16,460 Da
NCBI Official Full Name
eukaryotic translation initiation factor 1A, X-chromosomal
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 1A, X-linked
NCBI Official Symbol
EIF1AX??[Similar Products]
NCBI Official Synonym Symbols
EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1
??[Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 1A, X-chromosomal
UniProt Protein Name
Eukaryotic translation initiation factor 1A, X-chromosomal
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 4C; eIF-4C
Protein Family
Eukaryotic translation initiation factor
UniProt Gene Name
EIF1AX??[Similar Products]
UniProt Synonym Gene Names
EIF1A; EIF4C; eIF-1A X isoform; eIF-4C??[Similar Products]
UniProt Entry Name
IF1AX_HUMAN
NCBI Summary for EIF1AX
This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq, Jul 2008]
UniProt Comments for EIF1AX
Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.
Research Articles on EIF1AX
1. The finding of this study suggested that an SF3B1 or EIF1AX mutation is present in a substantial subset of primary LMNs underscores that these tumors genetically resemble uveal melanoma and are different from cutaneous melanoma at the genetic level.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
