Product Name
EIF1AX, Polyclonal Antibody
Popular Item
Full Product Name
EIF1AX Polyclonal Antibody
Product Synonym Names
EIF1AX; EIF1A; EIF1AP1; EIF4C; eIF-1A; eIF-4C; eukaryotic translation initiation factor 1A, X-linked
Product Gene Name
anti-EIF1AX antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
10-144
3D Structure
ModBase 3D Structure for P47813
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 10-144 of human EIF1AX (NP_001403.1).
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20°C
Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-EIF1AX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EIF1AX antibody
This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA
Product Categories/Family for anti-EIF1AX antibody
Polyclonal
Applications Tested/Suitable for anti-EIF1AX antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EIF1AX antibody
WB: 1:200 - 1:2000
IHC: 1:100 - 1:200
Western Blot (WB) of anti-EIF1AX antibody
Western blot analysis of extracts of various cell lines, using EIF1AX antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry (IHC) of anti-EIF1AX antibody
Immunohistochemistry of paraffin-embedded human stomach using EIF1AX antibody at dilution of 1:100 (40x lens).
Immunohistochemistry (IHC) of anti-EIF1AX antibody
Immunohistochemistry of paraffin-embedded mouse brain using EIF1AX antibody at dilution of 1:100 (40x lens).
NCBI/Uniprot data below describe general gene information for EIF1AX. It may not necessarily be applicable to this product.
NCBI Accession #
P47813.2
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UniProt Primary Accession #
P47813
[Other Products]
UniProt Secondary Accession #
Q0VGC2; Q5JPS5; Q5JPS6; B2R5U5[Other Products]
UniProt Related Accession #
P47813[Other Products]
Molecular Weight
Calculated: 16kDa
Observed: 16kDa
NCBI Official Full Name
Eukaryotic translation initiation factor 1A, X-chromosomal
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 1A, X-linked
NCBI Official Symbol
EIF1AX??[Similar Products]
NCBI Official Synonym Symbols
EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1
??[Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 1A, X-chromosomal; eIF-1A X isoform; eukaryotic translation initiation factor 4C; Putative eukaryotic translation initiation factor 1A; eukaryotic translation initiation factor 1A, X chromosome
UniProt Protein Name
Eukaryotic translation initiation factor 1A, X-chromosomal
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 4C; eIF-4C
Protein Family
Eukaryotic translation initiation factor
UniProt Gene Name
EIF1AX??[Similar Products]
UniProt Synonym Gene Names
EIF1A; EIF4C; eIF-1A X isoform; eIF-4C??[Similar Products]
UniProt Entry Name
IF1AX_HUMAN
NCBI Summary for EIF1AX
This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq, Jul 2008]
UniProt Comments for EIF1AX
EIF1AX: Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. Belongs to the eIF-1A family
Protein type: Translation initiation; RNA-binding; Translation
Chromosomal Location of Human Ortholog: Xp22.12
Cellular Component: cytosol
Molecular Function: protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity
Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression
Research Articles on EIF1AX
1. The effect of an EIF1AX mutation is estimated to be a decreased odds of metastasis in uveal melanoma.
Precautions
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Disclaimer
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