Product Name
TUBB2B, Polyclonal Antibody
Full Product Name
TUBB2B Antibody (N-term)
Product Synonym Names
Tubulin beta-2B chain; TUBB2B
Product Gene Name
anti-TUBB2B antibody
[Similar Products]
Antibody/Peptide Pairs
TUBB2B peptide (MBS9218739) is used for blocking the activity of TUBB2B antibody (MBS9202349)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
12-39
3D Structure
ModBase 3D Structure for Q9BVA1
Species Reactivity
Human (Predicted Reactivity: Chicken, Monkey, Mouse, Rat, Bovine, Xenopus, Pig)
Specificity
This TUBB2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human TUBB2B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-TUBB2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TUBB2B antibody
The protein encoded by this gene is a beta isoform of
tubulin, which binds GTP and is a major component of microtubules.
This gene is highly similar to TUBB2A and TUBB2C. Defects in this
gene are a cause of asymmetric polymicrogyria. [provided by
RefSeq].
Product Categories/Family for anti-TUBB2B antibody
Signal Transduction
Applications Tested/Suitable for anti-TUBB2B antibody
Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TUBB2B antibody
IF~~1:10~50
WB~~1:1000
Immunofluorescence (IF) of anti-TUBB2B antibody
Confocal immunofluorescent analysis of TUBB2B Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot (WB) of anti-TUBB2B antibody
TUBB2B Antibody (N-term) western blot analysis in HepG2,Hela,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TUBB2B antibody detected the TUBB2B protein (arrow).
Immunohistochemistry (IHC) of anti-TUBB2B antibody
TUBB2B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TUBB2B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
NCBI/Uniprot data below describe general gene information for TUBB2B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_821080.1
[Other Products]
NCBI GenBank Nucleotide #
NM_178012.4
[Other Products]
UniProt Primary Accession #
Q9BVA1
[Other Products]
UniProt Secondary Accession #
A8K068[Other Products]
UniProt Related Accession #
Q9BVA1[Other Products]
NCBI Official Full Name
tubulin beta-2B chain
NCBI Official Synonym Full Names
tubulin, beta 2B class IIb
NCBI Official Symbol
TUBB2B??[Similar Products]
NCBI Official Synonym Symbols
PMGYSA; bA506K6.1
??[Similar Products]
NCBI Protein Information
tubulin beta-2B chain
UniProt Protein Name
Tubulin beta-2B chain
UniProt Gene Name
TUBB2B??[Similar Products]
UniProt Entry Name
TBB2B_HUMAN
NCBI Summary for TUBB2B
The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq, Mar 2010]
UniProt Comments for TUBB2B
TUBB2B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB2B is implicated in neuronal migration. Defects in TUBB2B are a cause of polymicrogyria asymmetric (PMGA). PMGA is a malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination. Belongs to the tubulin family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 6p25
Cellular Component: microtubule; cytoplasm; nucleus
Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; neuron migration; transmembrane transport; microtubule-based process
Disease: Polymicrogyria, Symmetric Or Asymmetric
Product References and Citations for anti-TUBB2B antibody
Xu, W., et al. Mol. Cancer Ther. 8(12):3318-3330(2009)
Jaglin, X.H., et al. Nat. Genet. 41(6):746-752(2009)
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Cucchiarelli, V., et al. Cell Motil. Cytoskeleton 65(8):675-685(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Research Articles on TUBB2B
1. The association of polymicrogyria with thin or absent corpus callosum, dysmorphic basal ganglia, brainstem and vermis hypoplasia is highly likely to result from mutations in TUBB2B.
Precautions
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